| Lecturers: | Prof. Dr. Ilka Diester, PD Dr. Philippe Coulon, Prof. Dr. Barbara Di Ventura, Dr. Franziska Schneider |
| Lecture: | IMBIT, Georges-Köhler-Allee 201, 79110 Freiburg |
| Practical Course |
Experiments 1-3: IMBIT, Georges-Köhler-Allee 201, 79110 Freiburg, Lab room
Experiment 4: Schänzlestrasse 1, Di Ventura Lab, room 04.023 (4thfloor), teaching room 045 (ground floor) |
| Time: | SS |
| Participants: | max. 9 |
| Group size: | max. 3 students per setup |
Wahlmodul 31: Optophysiology
Description
Aim of this practical course is to provide students with an overview and introduction to standard optogenetic approaches. These include four experiments: 1) Histological analysis of cryosections with a focus on cortical areas 2) Two-Photon Ca2+ Imaging and Optogenetics in Organotypical Slice Cultures 3) Extracellular electrophysiology combined with optogenetics 4) Gibson assembly-based cloning of a reporter system regulated by a light-inducible synthetic transcription factor (TF) in mammalian cells.
Impressions from SS 2022
